Players will enter the Chalice Dungeon together as a team, striving to destroy the monstrous denizens within, but all partnerships come to an end. In Bloodborne, The Card Game, 3 to 5 Hunters work together to get through the Dungeon and collect Yharnam's blood before facing off against the horrible final boss.

Hunters engage in battle with monsters, secretly selecting an action card to play simultaneously. Discussion is allowed, even encouraged, but players aren't bound by any promise they make. As they advance deeper into the Dungeon, they will collect the blood of their fallen opponents. If the team can survive the final boss, the Hunter with the most trophies will be named the victor.